C4-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens
نویسندگان
چکیده
منابع مشابه
Mutant strains of clover rhizobium (Rhizobium trifolii) that form nodules on soybean (Glycine max).
Mutant strains of Rhizobium trifolii that induce nitrogenase activity in the free-living state were isolated. These strains, unlike the wild type, nodulated soybean and mungbean plants, producing effective nodules. One of the strains, strain DT72, also nodulated clover but the nodules were ineffective. Strain DT125 consumed hydrogen gas from the gas phase, which can be coupled to nitrogenase ac...
متن کاملCharacterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodules.
Mutants of Rhizobium meliloti SU47 with defects in the production of the Calcofluor-binding expolysaccharide succinoglycan failed to gain entry into alfalfa root nodules. In order to define better the polysaccharide phenotypes of these exo mutants, we analyzed the periplasmic oligosaccharide cyclic (1-2)-beta-D-glucan and lipopolysaccharide (LPS) in representative mutants. The exoC mutant lacke...
متن کاملSymbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum.
The transport of succinate was studied in bacteroids of an effective, streptomycin-resistant strain (GF160) of Rhizobium leguminosarum. High levels of succinate transport occurred, and the kinetics, specificity, and sensitivity to metabolic inhibitors were similar to those previously described for free-living cells. The symbiotic properties of two transposon (Tn5)-mediated C4-dicarboxylate tran...
متن کاملGenome sequence of the Trifolium rueppellianum -nodulating Rhizobium leguminosarum bv. trifolii strain WSM2012.
Rhizobium leguminosarum bv. trifolii WSM2012 (syn. MAR1468) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an ineffective root nodule recovered from the roots of the annual clover Trifolium rueppellianum Fresen growing in Ethiopia. WSM2012 has a narrow, specialized host range for N2-fixation. Here we describe the features of R. leguminosarum bv. trifolii stra...
متن کاملCitrate synthase mutants of Sinorhizobium fredii USDA257 form ineffective nodules with aberrant ultrastructure.
The tricarboxylic acid (TCA) cycle plays an important role in generating the energy required by bacteroids to fix atmospheric nitrogen. Citrate synthase is the first enzyme that controls the entry of carbon into the TCA cycle. We cloned and determined the nucleotide sequence of the gltA gene that encodes citrate synthase in Sinorhizobium fredii USDA257, a symbiont of soybeans (Glycine max [L.] ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1981
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.78.7.4284